Researches AntiBacter Research Group

AntiBacter Research Group



Focus of the Lab
  1. Investigation of the zoonotic potential of avian and bovine pathogenic E. coli
  2. CRISRP-CAS mediated genome excision to target virulence/antibiotic resistance genes
  3. Silencing of the bacterial toxins/virulent genes of E. coli via synthetic sRNA
  4. Phage therapy; molecular characterization of bacteriophages to use them against bacteria
for further details of the project please visit www.antibacter.com
Group Lead
Dr. Fazal Adnan 

Assistant Professor
Industrial Biotechnology, ASAB NSUT
Tel: +92 51 90856142, Cell: 03333122939
Email: fazliadnan@gmail.com, adnanfazal@asab.nust.edu
Specialization:  Molecular Microbiology, RNA biology
Education: PhD Molecular Microbiology, Justus-Leibig University of Giessen, Germany

Lab Members 
PhD Scholars 
  1. Amna Jalil
  2. Sidra Hafeez
  3. Hira Niaz
  4. Gul Naz
  5. Maria Kanwal
MS Students 
  1. Andleeb Khizar
  2. Bakhtawar Nawaz
  3. Tehreem Nadeem
  4. Zamar
  5. Ahmed Hafeez
  6. Anza
  7. Muneeb Ul Hassan
Research Associate 
  1. Quratulain Farooq
  2. Taj Wali Khan
  3. Arsalan Khan
Current Projects
As PI
  1. CRISPR-CAS3 mediated whole genome excision of the Avian Pathogenic E. coli (APEC); a novel strategy to combat colibacillosis in poultry birds
  2. Investigation of the antibiotic resistance and zoonotic potential of Avian Pathogenetic E. coli (APEC) and Mammary Pathogenic E. coli (MPEC) from poultry and bovine source
  3. Application of phage cocktail as preventative and therapeutic approach to combat E. coli  infections​ in  human
  4. Pharmacoinformatic screening of the MDR pathogenic E. coli strains and devising an antibacterial peptide based therapy against these MDR strains
As Co-PI
  1. Combating biofouling in the reverse osmosis membrane via Rhamnolipids; a novel greener alternative    approach
Research Funding
  1. CRISPR-CAS3 mediated whole genome excision of the Avian Pathogenic E. coli (APEC); a novel          strategy to combat colibacillosis in poultry birds”.  This project of  3.1 million PKR is awarded as PI  under the HEC- NRPU program by HEC, Pakistan. Grant duration 2019 to 2021.
  2. Combating biofouling in the reverse osmosis membrane via Rhamnolipids; a novel greener alternative    approach”. This project of 2.7 million PKR is awarded as Co-PI under the HEC- NRPU program by        HEC,Pakistan. Grant duration 2020 to 2022.
  3. Silencing of the antibiotic resistance mecA gene via synthetic non-coding RNAs in the Staphylococcus    aureus”. This project of 0.5 million PKR is awarded under the HEC- SRGP program by the HEC,          Pakistan.Grant duration 2016 to 2018.

Publications:

1) F. Adnan*, Amna Jalil, Tahir Ahmed, Afra Rahman, Nawal Dawood, Muhmmad Faisal, Sebastian Guenther. (Aug. 2021) TRAP-T transporter TakP; a key player in resistance against selenite-induced oxidative stress in Rhodobacter sphaeroides. J. Microbiological Research. https://doi.org/10.1016/j.micres.2021.126828 (IF 5.45)

2) Nafeesa Shaheen, Amna Jalil, Fazal Adnan* and Rao Arslan Khushnood, (Feb. 2021). Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO3 precipitation in bio‐based cementitious composites, J. Microbial Biotechnology, doi.org/10.1111/1751-7915.13752 (IF 5.85) 

3) Iqbal Nisa, Muhammad Qasim, Arnold Driessen, Fazal Adnan, Hazir Rahman (May 2021). Virulence profiling of Shigella flexneri and emergence of serotype 2b as a highly virulent shigellosis causing strain in Pakistan, Infection, Genetics and Evolution, https://doi.org/10.1016/j.meegid.2021.104922 (IF 3.45)

4) Syed Ali Rizwan, Hasnain Khan, Thomas A Bier, F. Adnan* (2017), Use of Effective Micro-organisms (EM) Technology and Self-Compacting Concrete (SCC) Technology Improved the Response of Cementitious Systems. J. Const. & Build. Mat. Vol. 152, 642-650. (IF 6.14)

5) Qurrat ul ain, Amir Ali Shah, Samiullah Khan, Fazal Adnan, and Malik Badshah. (Dec. 2019) Starved Spirodela polyrhiza and Saccharomyces cerevisiae: A Potent Combination for Sustainable Bioethanol Production” Biomass Conv Bioref doi:10.1007/s13399-019-00540-z. (IF 5.02)

6) Fazal Adnan, L. Weber and G. Klug, (2015) The sRNA SorY confers resistance during photooxidative stress by affecting a metabolite transporter in Rhodobacter sphaeroides. RNA biology 12:5, 569-577. (IF 5.65)

7) Nuss, A.M., F. Adnan, L. Weber, B.A. Berghoff, J. Glaeser & G. Klug, (2013) DegS and RseP homologous proteases are involved in singlet oxygen dependent activation of RpoE in Rhodobacter sphaeroides. PloS one 8: e79520. (IF 3.42) 

8) Fatma Abdelrahman, Maheswaran Easwaran, Stephanie Lynch, Tolulope J. Oduselu, Fazal Mehmood Khan, Akomolafe Ayobami, Fazal Adnan & Ayman El-Shibiny (Jan. 2021), Phage-encoded endolysins. Journal Antibiotics, doi.org/10.3390/antibiotics10020124 (IF 4.65) 

9) Sayed Abdullah, Fazal Adnan*, Amna Jalil, Quratul Ain, Muhammad Qasim, Dilawar Khan. Silencing of Culrin via an M13 Phagemid mediated sRNA expression reduces virulence in the Avian Pathogenic E. coli (APEC) (minor revision submitted to Current Microbiology Aug. 2021) (IF 2.42)

10) Abubakar Siddique, Sara Azim, Fazal Adnan, Muhammad Imran, Abdur Rahman*. (Aug. 2021) Lactobacillus reuteri and Enterococcus faecium from poultry gut reduce mucin adhesion and biofilm formation of cephalosporin and fluoroquinolone-resistant Salmonella enterica. (Accepted in Animals journal) (IF 2.75)

11) Roha Tariq, Usama Ahmed Khalid, Fazal Adnan & Muhammad Qasim, (Mar. 2021) Photodynamic therapy: A rational approach toward COVID-19 management, Journal of Exp. Res. in Pharmacology, 10.14218/JERP.2020.00036

12) Aifa Nemat, Ishaq N. Khan, Fazal Adnan, & Mudassir Iqbal (Nov. 2020) Synthesis, anticancer evaluation and molecular docking studies of methotrexate’s novel Schiff base derivatives against malignant glioma cell lines, Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2020.1844053 (IF 3.23)

13) Ayesha Talib, KN Manzoor, Ijaza Ahmed, Fazal Adnan & Abid ali khan (Aug. 2020) Encapsulated Virgin Coconut Oil (VCO) as a Nanoscale in vitro Solution against Multiple Drug Resistant (MDR) Staphylococcus aureus, Micro and Nano letters. DOI: 10.1049/mna2.12001. (IF 1.2)

14) M. Faisal, Ihsan ullah, Harvey Wintersa, Farhana Maqboolb, Fazal Adnan (2019). Tannic acid Treatment to Deter Microbial Biofouling in Flow Cell System and on RO Membrane in Drip Flow Reactor. Desalination and Water Treatment doi: 10.5004/dwt.2019.24767. (IF 1.25)

15) Tahir A, F. Adnan, M. Nadeem (2018). Assessment of the risk for human health of Enterovirus and Hepatitis A virus in clinical and water sources from three metropolitan cities of Pakistan. Annals of Agri. & Env. Med, doi: 10.26444/aaem/99590. (IF 1.44)

16) M. Adil, F. Adnan, J. Ahmed and Ishaq N. Khan (2018) Association of Metronidazole with Cancer: A Potential Risk Factor or Inconsistent Deductions? Current Drug Metabolism doi: 10.2174/1389200219666180329124130. (IF 3.74)

17) Tahir Ahmad, Najma Arshad, F. Adnan, Najam-us-Sahar Sadaf Zaidi (2016). Prevalence of rotavirus, adenovirus, hepatitis A virus and enterovirus in water samples collected from different region of Peshawar, Pakistan. Annals of Agri. & Env. Med, vol. 23, No 4, 544-548. (IF 1.44) 

18) I. Haq*, S. Ali, M.M. Javed, A. Saleem, F. Adnan and M.A. Qadeer (2010) IIB GCU, Pakistan. Production of alpha amylase from a randomly induced Bacillus amyloliquefaciens and its application as a desizer in textile industry, Pak. J. Bot., 42(1): 473-484 (IF 1.12)

19) I. Haq*, M.M. Javed, and F. Adnan (2010) IIB GCU, Pakistan. Kinetics & thermodynamic studies of alpha amylase from Bacillus licheniformis mutant. Pak. J. Bot., 42(5). (IF 1.12)